![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800050:Design
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-YahK-SAV2585
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 4316
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4316
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 4316
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 4316
Illegal NgoMIV site found at 3852
Illegal NgoMIV site found at 4516
Illegal NgoMIV site found at 4545
Illegal NgoMIV site found at 4590
Illegal NgoMIV site found at 5394
Illegal NgoMIV site found at 5508
Illegal AgeI site found at 4201
Illegal AgeI site found at 4252
Illegal AgeI site found at 4327
Illegal AgeI site found at 4441 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5781
Illegal BsaI site found at 6849
Illegal SapI.rc site found at 767
Design Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.
Source
None