Composite

Part:BBa_K4800050:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-YahK-SAV2585


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4316
    Illegal NgoMIV site found at 3852
    Illegal NgoMIV site found at 4516
    Illegal NgoMIV site found at 4545
    Illegal NgoMIV site found at 4590
    Illegal NgoMIV site found at 5394
    Illegal NgoMIV site found at 5508
    Illegal AgeI site found at 4201
    Illegal AgeI site found at 4252
    Illegal AgeI site found at 4327
    Illegal AgeI site found at 4441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5781
    Illegal BsaI site found at 6849
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.



Source

None

References